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. 2017 Aug 15;144(16):2896–2906. doi: 10.1242/dev.147660

Fig. 3.

Fig. 3.

Canonical DAF-3-binding sites are not required for the effect of DAF-7/TGFβ on lag-2 DTC expression. (A) Positions of three canonical DAF-3-binding sites (Thatcher et al., 1999) within 3 kb upstream of the lag-2 ATG. (B) Distal gonad arms from two animals, each expressing two reporters: the wild-type single-copy naSi8 GFP-PH reporter; and naIs96, an integrated reporter in which mCherry-PH is driven by a lag-2 promoter, in which three canonical DAF-3-binding sites were mutated as in A. The expression of each reporter was measured in individual wild-type (left) and daf-7(e1372) (right) animals. Scale bars: 5 μm. (C) Quantification of GFP (top) and mCherry (bottom) in the DTC in the different genetic backgrounds. Mean pixel intensity (arbitrary units), measured in the DTC as described in the Materials and Methods. The boxes indicate the minimum-maximum range of samples quantified. **P<0.01, ***P<0.001, two-tailed Student's t-test. n≥15 animals, one DTC scored per animal. Error bars represent s.e.m.