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. 2017 Aug 15;144(16):2896–2906. doi: 10.1242/dev.147660

Fig. 5.

Fig. 5.

The conserved (Cons) sequence is required for the response of lag-2 to DAF-7/TGFβ signaling in the DTC. (A) Positions of the DAF-3-binding site (DBS) and conserved (Cons) sequences. (B-G) Expression of lag-2 (1 kb, low-copy) GFP-PH reporters in the DTC of wild-type, daf-7(e1372) and daf-7(e1372);daf-3(e1376) animals. Reporters are lacking (B-C) DBS sequence (1kbΔDBS), naIs100; (D-E) conserved (Cons) sequence (1kbΔCons), naIs102; or both (1kbΔboth, F-G), naIs106. (C,E,G) Quantification of GFP expression; mean pixel intensity (arbitrary units), measured in the DTC as described in Materials and Methods. The boxes indicate the minimum-maximum range of samples quantified. Scale bars: 5 µm. n.s. indicates P>0.05, *P<0.05, **P<0.01, two-tailed Student's t-test. n≥15 animals, one DTC scored per animal. Error bars represent s.e.m. In all cases, P>0.05 for wild type versus double mutants.