Fig. 7.

A working model for the regulation of late larval DTC-expressed lag-2. (Top) Under favorable conditions, DAF-7/TGFβ pathway activity inhibits repressor activity of the DAF-3/DAF-5 complex, possibly by interfering with complex formation or stability. lag-2 expression is driven by positive regulators, including HLH-2, which binds the E-box (green), and unknown regulators that bind other conserved sequences (yellow) shown in Fig. S7. DAF-3 may bind both the DBS (blue) and the Cons (red) sequences under favorable conditions, but it is not active. (Bottom) Under unfavorable conditions, the DAF-3/DAF-5 complex is active and represses lag-2 expression, possibly by interfering with non-HLH-2 activators. See Discussion for details.