Fig. 1.

Genomic characteristics of mouse Plcz1^em1Jparr and Plcz1^em2Jparr mutant alleles. (A) Gene structure of the mouse Plcz1 gene (NM_054066.4) and target sequences (red lines) for CRISPR/Cas9. Exons are represented by vertical bars (unfilled, untranslated regions; filled, coding regions). (B,C) Comparison of genomic sequences from wild-type Plcz1 (Plcz1^WT) allele and two mutant Plcz1 alleles harbouring nucleotide deletions (deleted sequence shaded grey in Plcz1^WT sequence). Capital letters correspond to exonic sequences and lower case letters to intronic sequences. (D) Typical result from a genotyping PCR reaction for both Plcz1 mutant lines: HET, Plcz1^+/em1Jparr or Plcz1^+/em2Jparr; MUT, homozygote Plcz1^em1Jparr or Plcz1^em2Jparr; WT, homozygote Plcz1^WT.