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. 2017 Aug 15;144(16):2914–2924. doi: 10.1242/dev.150227

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© 2017. Published by The Company of Biologists Ltd

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Fig. 3. — Characterization of sperm from homozygote Plcz1^em1Jparr and Plcz1^em2Jparr mice. (A) Histological analysis of testes sections stained with Hematoxylin/Eosin. (B) Density plots of sperm stained with SYBR14 (live sperm) and propidium iodide (PI; dead sperm) and analysed by flow cytometry. A total of 10,000 events were collected for each group. (C) Quantification of data from B. Representative stained sperm were obtained using an Olympus BX40 microscope. (D-G) Analysis of sperm motility parameters using computer-assisted sperm analysis (CASA). (H) An acrosome reaction was induced either by treatment with progesterone or ionomycin, and reacted sperm were assessed by lack of reactivity towards fluorescently labelled lectin PNA. Representative images of reacted and unreacted sperm are shown. All data are presented as average±s.e.m. of four different animals. There are no statistically significant differences.