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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Kidney Int. 2017 Jan 25;91(5):1104–1114. doi: 10.1016/j.kint.2016.10.034

Figure 5. Effects of chronic administration of αKlotho on cardiac remodeling in chronic kidney disease (CKD) mice.

Figure 5

Animal experimental design was shown in Figure 3a. At 16 weeks after surgery, hearts were harvested for histology, immunoblot, and Immunohlstochemlstry. (a) Cardiac hypertrophy In CKD mice. Upper panel shows representative gross macrographs of hearts. Bottom panel shows a summary of ratio of heart weight to body weight for the examined mice, (b) Cardiac fibrosis in CKD mice. Upper panel shows representative macrographs of sagittal sections (trichrome stain). Middle panel shows representative micrographs of left ventricular sections (trichrome stain). Bottom panel is a summary of semiquantification of trichrome-positive area to whole heart section performed using ImageJ software. Data are expressed as means ± SD of 4 mice from each group, (c) Hypertrophic cardiomyocytes in CKD mice. Left panel shows representative micrographs of left ventricular sections stained with wheat germ agglutinin. Right panel is a summary of cardiomyocyte size calculated using ImageJ software, (d) Hypertrophic and fibrotic markers in the heart. Left panel shows representative immunoblotsfor α-actinin, α-smooth muscle actin (α-SMA), and β-actin protein. Right panel is a summary of normalized protein quantification from all examined immunoblots. Data are expressed as means ± SD of 4 mice from each group. Statistical significance was assessed by 1-way analysis of variance followed by Student-Newman-Keuls test and accepted when: *P < 0.05, **P < 0.01 between 2 groups.