Figure 2.

Expression of miR-21 is affected by wound fluids in cells from the basal/epithelial, HER2 OE and luminal subtypes of breast cancer. Expression of miR-21 did not change in the BT-549 cell line, and was decreased in the MDA-MB-468 and MCF7 cell lines following incubation with WF and RT-WF. In the SK-BR-3 cell line, miR-21 expression was increased after WF and RT-WF stimulation. Graphs show the relative mRNA fold change normalized to untreated cell lines. All samples were assessed in triplicate and analyzed using one-way analysis of variance with Tukey's post-hoc test. P<0.05 was considered to indicate a statistically significant difference. HER2 OE, human epidermal growth factor receptor 2 overexpression; WF, post-operative wound fluid; RT-WF, post-operative and post-intraoperative radiotherapy wound fluid. *P<0.05; **P<0.01; ***P<0.001.