Figure 3.

Expression of miR-155 is highly affected by wound fluids in all subtypes of analyzed breast cancer cells. Expression of miR-155 was decreased significantly in the BT-549, MDA-MB-468 and MCF7 cell lines. In the MCF7 cell line, an increase in miR-155 expression following RT-WF stimulation was also identified compared with WF stimulation. By contrast, the miR-155 expression level was increased in the SK-BR-3 cell line stimulated with WF. Only low expression stimulation was observed in the RT-WF group, but it was not statistically significant compared with the control. Graphs show the relative mRNA fold change normalized to untreated cell lines. All the samples were assessed in triplicate and analyzed using one-way analysis of variance with Tukey's post-hoc test. P<0.05 was considered to indicate a statistically significant difference. HER2 OE, human epidermal growth factor receptor 2 overexpression; WF, post-operative wound fluid; RT-WF, post-operative and post-intraoperative radiotherapy wound fluid. *P<0.05; **P<0.01; ***P<0.001.