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. 2017 Aug 23;51(4):1055–1066. doi: 10.3892/ijo.2017.4101

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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YAP knockdown in GC-MSC inhibits its promoting effect on the growth of gastric cancer cells in vitro. (A) Colony formation assay was performed to analyze the proliferation ability of gastric cancer cells (SGC-7901, HGC-27 and MGC-803) with different treatments. The colony number was counted. Blank gastric cancer cells, untreated gastric cancer cells; sh-Ctrl CM group, gastric cancer cells were treated with the 72 h conditioned medium of GC-MSCs transfected with control lentiviral; sh-YAP CM group gastric cancer cells, gastric cancer cells were treated with the 72 h conditioned medium of GC-MSCs transfected with ShYAP lentiviral. ^**P<0.01, ^***P<0.001. (B) Immunofluorescence analysis revealed that the expression level of Ki67 in blank group, sh-Ctrl CM group and sh-YAP CM group. Magnification, ×200; scale bar, 100 µm. (C) Western blotting for protein levels of Bax, Bcl2 and PCNA. (D) The apoptosis of gastric cancer cells was analyzed by using flow cytometry.