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. 2017 Aug 10;51(4):1089–1103. doi: 10.3892/ijo.2017.4095

Figure 6.

Figure 6

The effects of chrysophanol nanoparticle on ROS, p53 and apoptosis. (A) Upper, LNCap cells were treated with 90 μM chrysophanol nanoparticle for 24 h with or without ROS scavenger of NAC (1 mM) pre-treatment for 1 h. Then, p53 and p27 protein levels were assessed using western blot analysis. Lower, LNCap cells were treated with 90 μM chrysophanol nanoparticle in the presence or absence of p53 inhibitor, PIF-α (30 μM), for 24 h. Then, all cells were harvested for p53 and p27 assessment through western blot analysis. Viability assay of LNCap cells using (B) NAC (1 mM) and (C) p53-inhibitor (PIF-α, 30 μM) with 90 μM chrysophanol nanoparticle was conducted via MTT analysis. (D) LNCap cells were exposed to 90 μM chrysophanol nanoparticle and 30 μM PIF-α in single or double treatments for 24 h. Then, flow cytometry analysis was carried out to evaluate apoptosis. Data are shown as mean ± SEM. *P<0.05, **P<0.01 and ***P<0.001.