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. 2017 Aug 10;51(4):1089–1103. doi: 10.3892/ijo.2017.4095

Figure 7.

Figure 7

Chrysophanol nanoparticle promotes the release of Cyto-c into the cytoplasm. (A) LNCap cells were treated with 90 μM chrysophanol nanoparticle for 24 h, and then immunofluorescent analysis was used to evaluate Cyto-c expression levels. (B) LNCap cells were treated with 90 μM chrysophanol nanoparticle for 24 h with or without NAC (1 mM) pre-treatment for 1 h, followed by fluorescent intensity analysis of Cyto-c. (C) Chrysophanol nanoparticle (70, 90 and 110 μM) was administered to LNCap cells for 24 h. Then, Apaf-1 and Cyto-c expression levels were evaluated using western blot analysis. (D) LNCap cells were treated with 90 μM chrysophanol nanoparticle for 0, 6, 12, 24, 36 and 48 h, followed by Apaf-1 and Cyto-c measurement using western blot assays. Data are shown as mean ± SEM. *P<0.05, **P<0.01 and ***P<0.001.