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. 2017 Aug 30;51(4):1320–1330. doi: 10.3892/ijo.2017.4108

Figure 3.

Figure 3

Downstream target genes regulated by HER2, mutant PIK3CA and both of HER2 and mutant PIK3CA. (A) The resulted number of probe sets that upregulated, downregulated, or no change in MCF10A/HER2, MCF10A/PIK3CA-H1047R and MCF10A/HER2/PIK3CA-H1047R models after compared to control cell model MCF10A/LacZ. (B) Venn diagram shows common and differential expressed probe sets among three cell models. Black: upregulated; gray: downregulated. (C) The top 5 upregulated and top 5 downregulated common downstream gene targets in three comparisons of MCF10A/HER2 vs. MCF10A/LacZ; MCF10A/PIK3CA-H1047R vs. MCF10A/LacZ, and MCF10A/HER2/PIK3CA-H1047R vs. MCF10A/LacZ control. (D) Specific deregulated genes were identified in MCF10A/HER2/PIK3CA-H1047R cells, but not in either MCF10A/HER2 or MCF10A/PIK3CA-H1047R cells, after compared to MCF10A/LacZ control cells. Top 10 up-/down-regulated genes are shown here. Probe sets with log2 expression values <4 for any two cell lines compared were excluded.