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Silencing of Wnt3a expressions induces G0/G1 cell cycle arrest by regulating cell cycle regulatory proteins in MHcc97H and SK-Hep-1 cells. Cell cycle profiles were measured with flow cytometry following Wnt3a-siRNA interference treatment for 24 h (A). Western blotting was performed to detect c-Myc, p21, p27, and cyclin D1 expression in MHcc97H and SK-Hep-1 cells; GAPDH was used as a loading control (B).
