Table 7. Comparison of C. pecorum LAMP and qPCR for organism detection using rapidly processed swab samples and their DNA extracts.

Sample	LAMPa result for swab suspension	qPCRb result for swab suspension	LAMP result for DNA extract	qPCR result for DNA extract	LAMP result for “spiked” swab suspension	LAMP result for “spiked” DNA extract	qPCR result for “spiked” swab suspension	qPCR result for “spiked” DNA extract
K1 ocularc	NEG	NEG	0.00/83.49	NEG	NEG	–	NEG	–
K6 ocularc	NEG	NEG	21.00/83.23	3 × 103 (Ct 20)	NEG	–	NEG	–
K9 ocularc	NEG	NEG	25.45/83.39	287 (Ct 24)	NEG	–	NEG	–
K2 ocularc	NEG	NEG	NEG	NEG	NEG	–	NEG	–
R1 eye	25.45/83.39	222 (Ct 25)	20.15/83.27	750 (Ct 24)	–	–	–	–
R1 cloaca	30.00/83.34	NEG	NEG	NEG	11.15/83.47	12.15/83.42	5 × 103 (Ct 17)	1.5 × 103 (Ct 18)
K eye	27.00/83.15	NEG	0.00/83.35	NEG	–	–	–	–
Koala 2 eye	NEG	NEG	NEG	NEG	11.00/83.51	11.00/83.40	1.2 × 103 (Ct 19)	1.1 × 104 (Ct 15)
Koala 2 cloaca	27.30/83.77	116 (Ct 26)	21.30/83.49	375 (Ct 25)	–	–	–	–
Will Cloaca	0.00/83.77	NEG	NEG	NEG	12.00/83.45	11.00/83.34	1.5 × 103 (Ct 19)	8 × 103 (Ct 17)
23117 Eye	21.30/83.20	NEG	23.15/83.23	150 (Ct 25)	–	–	–	–
23117 Cloaca	22.00/83.29	NEG	24.00/83.15	90 (Ct 27)	–	–	–	–
Flyn eye	NEG	NEG	NEG	NEG	12.30/83.50	11.00/83.35	1.9 × 103 (Ct 18)	8.3 × 103 (Ct 16)
Tyke eye	NEG	NEG	NEG	NEG	12.00/83.44	10.45/83.40	1.3 × 103 (Ct 19)	9 × 103 (Ct 16)
Bill eye	NEG	NEG	NEG	NEG	12.15/83.49	10.45/83.34	1.2 × 103 (Ct 19)	1 × 104 (Ct 15)
Ray eye	NEG	NEG	NEG	NEG	12.45/83.49	11.00/83.40	4.7 × 103 (Ct 17)	1 × 104 (Ct 15)
Ray cloaca	NEG	NEG	NEG	NEG	12.15/83.43	11.00/83.30	700 (Ct 20)	9 × 103 (Ct 16)
Koala F Eye	NEG	NEG	NEG	NEG	11.45/83.45	11.00/83.35	1.3 × 103 (Ct 19)	1.1 × 104 (Ct 15)

Notes.

^aLAMP results are expressed as time to amplify (min) and melt (°C).

^bqPCR results are expressed as copies/reaction and Ct value.

^cRNA Later swabs.