Table 2. Growth conditions for each of the four species used during this study.

Species	Plate Media	Days for Growth	Broth Media	Days for Growth	Shaker	Environment
Histophilus somni	Columbia agar	1–2	BHI	1–2	N/A	Anaerobic
Mycoplasma bovis	PPLO	5	PPLO	5	N/A	5% CO2
Mannheimia haemolytica	Sheep’s blood agar	1–2	BHI	1–2	250 rpm	Facultative
Pasteurella multocida	Sheep’s blood agar	1–2	BHI	1–2	250 rpm	Facultative

BHI, brain–heart infusion; PPLO, pleuropneumonia-like organism.