Table 3. Primers and PCR amplification protocols used in this study.

Species	Primer	Sequence (5′-3′)	Tm (°)
Histophilus somni	Lob1F	AAATGGAAACACCCAACCAA	56.3
	Lob1R	CAAACGCTTGATACGCTCA	58.4
Mycoplasma bovis	MB528F	CATGCGAAAACACTGCAACT	58.4
	MB528R	CCACGCATTCAGAATTGAAA	56.3
Mannheimia haemolytica [Alexander et al. 2008]	LktF	GCAGGAGGTGATTATTAAAGTGG	61
	LktR	CAGCAGTTATTGTCATACCTGAAC	61.2
Pasteurella multocida [Ulhah et al. 2009]	KMT1SPG	GCTGTAAACGAACTCGCCAC	62.4
	KMT1T7	ATCCGCTATTTACCCAGTGG	60.4
Positive control	16SF	GCTAACTCCGTGCCAGCAG	64.5
	16SR	CGTGGACTACCAGGGTATCTAATC	65.6