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. 2017 Jul 14;7(9):3091–3102. doi: 10.1534/g3.117.043521

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Copyright © 2017 Đermić et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) DNA degradation in γ-irradiated E. coli depends on RecA protein concentration and its rate of association with ssDNA. Bacterial cultures were divided into two counterparts; one served as a control (open symbols), while the other was irradiated with 400 Gy (closed symbols). The cultures contained [³H]thymidine-labeled chromosome; kinetics of their degradation were monitored during incubation at 37°. AB1157 (□,▪); ∆recA (○,●); lexA3 (▵,▴); recAo281 (+,); lexA3 recAo281 (▿,▾); recA730 (◊,♦); and recA730 lexA3 (×,⊗). Each value is a mean of three independent experiments, with error bars representing SD. (B) Survival of γ-irradiated bacteria. Wild-type strain AB1157 (▪) and its recA (●), lexA3 (h), recA_o281 (▾), lexA3 recA_o281 (▿), recA730 (♦), and recA730 lexA3 (◊) derivatives. Fraction survival is given as a fraction of the unirradiated control. Each value is a mean of three independent experiments, with error bars representing SD. ssDNA, single-stranded DNA; wt, wild-type.

Inline graphic — (A) DNA degradation in γ-irradiated E. coli depends on RecA protein concentration and its rate of association with ssDNA. Bacterial cultures were divided into two counterparts; one served as a control (open symbols), while the other was irradiated with 400 Gy (closed symbols). The cultures contained [³H]thymidine-labeled chromosome; kinetics of their degradation were monitored during incubation at 37°. AB1157 (□,▪); ∆recA (○,●); lexA3 (▵,▴); recAo281 (+,); lexA3 recAo281 (▿,▾); recA730 (◊,♦); and recA730 lexA3 (×,⊗). Each value is a mean of three independent experiments, with error bars representing SD. (B) Survival of γ-irradiated bacteria. Wild-type strain AB1157 (▪) and its recA (●), lexA3 (h), recA_o281 (▾), lexA3 recA_o281 (▿), recA730 (♦), and recA730 lexA3 (◊) derivatives. Fraction survival is given as a fraction of the unirradiated control. Each value is a mean of three independent experiments, with error bars representing SD. ssDNA, single-stranded DNA; wt, wild-type.