Figure 5.

casper mutants allow improved visualization of fluorescent reporter genes. (A) A fluorescent GFP reporter of a previously described tooth enhancer (Erickson et al. 2015) is only clearly visible in the lenses (white arrow) of wild-type fish. (B) A casper sibling with the same stable integration of the fluorescent reporter reveals more readily apparent oral (white filled arrowhead) and pharyngeal teeth (asterisk) domains, and clearly reveals a major previously unreported liver expression domain (black filled arrowhead). (C) GFP reporter activity during oral tooth development and replacement. (D) The left side of the liver expression domain in casper fish (right visible in B). Bars, 1 mm.