Table 1. Filtering results for STACKS pipeline.
Quality Filtering Steps | Number of ddRAD Loci | % |
---|---|---|
Populating observed ddRAD loci for 42 animals | 7,576,300 | 100.0 |
Removing ddRAD loci below the minimum depth of 5× | 7,456,091 | 98.4 |
Removing ddRAD loci below the log likelihood threshold of −8 | 1,989,000 | 26.3 |
Removing ddRAD loci below the minimum constraint of 32 (75%) individuals | 324,564 | 4.3 |
Putative biallelic SNP (MAF ≥0.05) | 98,166 | 1.3 |