Figure 4.

Plasmid-based overexpression of TSA2 partially recapitulates the effect of having two chromosomal copies of this gene. The entirety of the TSA2 locus was cloned into a CEN plasmid that was then transformed into the haploid progenitor (see Materials and Methods). Two independently generated TSA2 overexpressing strains were screened for hydrogen peroxide tolerance alongside the haploid progenitor and disomic strain. Shown are 95% confidence intervals for the MIC of the overexpressing strains as a percentage of the MIC of the aneuploid strain. These measurements are based on three biological replicates, which were each grown in three technical replicates.