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. 2017 Sep 5;12:6559–6580. doi: 10.2147/IJN.S144705

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© 2017 Yang et al. This work is published and licensed by Dove Medical Press Limited

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GC chromatograms of fatty acids in coffee oil (A) and algae oil (B) using a DB-WAX column by modifying temperature programming condition.

Notes: Helium was used as carrier gas. The oven temperature was programmed as follows: 185°C in the beginning, maintained for 1 min, increased to 230°C at 5°C/min, maintained for 8 min. Coffee oil (A) peaks: 1, palmitic acid methyl ester; 2, stearic acid methyl ester; 3, oleic acid methyl ester; 4, linoleic acid methyl ester; 5, linolenic acid methyl ester; 6, arachidic acid methyl ester; 7, gadoleic acid methyl ester; and 8, behenic acid methyl ester. Algae oil (B) peaks: a, myristic acid methyl ester; 1, palmitic acid methyl ester; 3, oleic acid methyl ester; 9, docosapentaenoic acid methyl ester; and 10, docosahexaenoic acid methyl ester.

Abbreviations: S, solvent peak; IS, internal standard; GC, gas chromatography.