Figure 6.

VitC increases TET1 expression and modulates α-KG levels and MDH2 activity. (a) CC9^mus, CC9^dunni and CC9^pahari ES cells were treated with VitC and 5-Aza, and TET1 and DNMT1 protein levels were measured by western blot. Actin protein levels were used to confirm equivalent loading and the precision plus all blue protein marker (Biorad, Gladesville, NSW, Australia) was used used to determine protein size. TET1 (b) and DNMT1 (c) expression was normalised to actin and expressed as the fold change in expression compared to vehicle-treated cells. α-KG levels increased as a result of VitC treatment (d) but not 5-Aza (e) whilst MDH2 activity decreased as a result of VitC treatment (f) but 5-Aza had no effect (g) when compared to non-treated cells from the same line with values represented as fold change to non-treated cells. *P<0.05; **P<0.01.