Fig. 2.

Representative images and HSPA1 gene expression of blastocysts exposed to heat stress with or without cryopreservation. Fresh-con: blastocysts incubated at 38.5°C for 6 h from 174 hpi (hours post insemination); Fresh-HS: blastocysts incubated at 41.0°C for 6 h from 174 hpi; Cryo-con: blastocysts incubated at 38.5°C for 6 h after thawing; Cryo-HS: blastocysts incubated at 41.0°C for 6 h after thawing. Scale bar, 200 μm. Relative gene expression (Fresh-con = 1) of HSPA1 gene (mean ± SE), Different letters above bars indicated P < 0.05. Modified from [85].