| Primers for RT-PCR |
|
| IGF2 |
5'ACCCTCCAGTTTGTCTGTGG3' |
349 |
60 |
|
5'GGTGACTCTTGGCCTCTCTG3' |
|
|
| PEG3 |
5'CTTCGCGGTCATTTCTGAGT3' |
282 |
60 |
| 5'TTGTCCTTGCCGTACATCTTC3' |
| ZAC1 |
5'GGGAAGAAGTACAACACCATGC3' |
249 |
63 |
| 5'CTGTGTGGACCACCAGGT3' |
| NDN |
5'GTGAARGATGTCATCGGCAG3' |
590 |
60 |
| 5'GTCCTCWGAGACACTGYTGC3' |
| DLK1 |
5'GTGACCAGTGCGTGACCTTT3' |
454 |
54 |
| 5'GCAGGTCTTGTCCATGAAGC3' |
| SGCE |
5'CCCGTTACCCTATCAAGCAG3' |
557 |
56 |
| 5'GGCAGCACATGATATAAGCG 3' |
| PEG10 |
5'TCAACCTTTTGGGGGCTGTT3' |
284 |
60 |
| 5'GGGGTAAGAAGAAGGGCCAC3' |
| IMPACT |
5'TGGCGAGGAGTGGTGTGTCA3' |
594 |
68 |
| 5'GGCATAGATGTTGTGGGTGG3' |
| MAGEL2 |
5'CTGATGGTGGTTCTGAGCCT3' |
257 |
60 |
| 5'CAGGACAATCATCTTGCTGG3' |
| SNRPN |
5'TGGGAAGGAGCAGCAAGGTG3' |
532 |
62 |
| 5'TGGTCAACTGATGGTGGCGG3' |
| PEG1/MEST |
5'CGCCGAGATCGTCTCCGCAG3' |
377 |
58 |
| 5'CTCCACGATGCTGGCCTGCTC3' |
| IGF2R |
5'CTTGGCGGACCGGCACTTCAACTACACCTCACTGA3' |
282 |
60 |
| 5'CCTGCGGCTGCGGTGCACACCCCCACACTGTAG3' |
| H19 |
5'GATGGTGCTACCCAGCTCAT3' |
231 |
60 |
| 5'CCTTCCAGAGCTGATTCCTG3' |
| GNAS |
5'GAAGGACAAGCAGGTCTACC3' |
675 |
60 |
| 5'GACCATGTTGTAGCTGCTG 3' |
| UBE3A |
5'GGAGTTGATGAGGGAGGTGTT3' |
635 |
58 |
| 5'TCTGTAGTTTCTTCTAGTGCTTGGA3' |
| GRB10 |
5'GAAGATGGGACAAGCAAAGT3' |
290 |
58 |
| 5'CTGGCACCAAGTAACCATCTG3' |
| GTL2 |
5'CCCACCAGCAAACAAAGCAAC3' |
174 |
60 |
| 5'CATCAAGGCAAAAAGCACATCG3' |
| CDKN1C |
5'AGAGCCTCGTGCTCAAAGAG3' |
137 |
60 |
| 5'TTTAAACACGAAACCGAACG3' |
| beta-actin |
5'CAGAAGGACTCGTACGTGGG3' |
200 |
60 |
| 5'TTGGCCTTAGGGTTCAGGG3' |
| XIST |
5'AGCATTGCTTAGCATGGCTC' |
365 |
60 |
| 5'TGGCTGTGACCGATTCTACC3' |
| VIMENTIN |
5'AAATCCAGGAGCTTCAGGCC3' |
501 |
60 |
| 5'CCTCTCCTTCCAGCAGCTTC3' |
| CYTOKERATIN18 |
5'CCGCATCGTTCTGCAGATTG3' |
551 |
60 |
| 5'CATATCGGGCCTCCACTTCC3' |
| OCT4 |
5'GGCAAACGATCAAGCAGTG3' |
317 |
60 |
| 5'TAATCCCAAAGGCCTGGTAC3' |
| NANOG |
5'GTGTTTGGTGAACTCTCCTG3' |
307 |
60 |
| 5'GGGAATTGAAATACTTGACAG3' |
| SOX2 |
5'GGTTGACATCGTTGGTAATTTATAATAGC3' |
88 |
60 |
| 5'CACAGTAATTTCATGTTGGTTTTTCA3' |
|
| Primers for Bisulfite PCR |
| Satellite I |
5'AATACCTCTAATTTCAAACT3' |
211 |
46 |
| 5'TTTGTGAATGTAGTTAATA3' |
| Satellite II |
5'CAACCCATAATCAATAAACTC3' |
297 |
46 |
| 5'GTTGAGGTAGTAGTTAGGTA3' |
| apha-satellite |
5'AATAATTCCACATTCCRTAAAACCC3' |
153 |
55 |
| 5'GATGTTTTYGGGGAGAGAGG3' |
| Art2 |
5'TTAAATTCAATTCAATCACTCAATCA3' |
224 |
55 |
| 5'TTTATGTGAAGAGTTGATTTATTGGA3' |
| H19 DMR |
5'TTTTGTGGATTATTGTGGTATT3' |
198 |
55 |
| 5'ATCTTAAACTAATCTCCCAACCC3' |
|
PEG3 DMR (1st) |
5'GGAGGAAGAAGTTGGAGTAGA3' |
– |
51 |
| 5'CCCCTACCCAAAATAATCAAC3' |
|
PEG3 DMR (2nd) |
5'GATATGTTTATTTTTGGTTGTTGG3' |
280 |
51 |
| 5'ACCCTAATCCCAAACTCCAACT3' |
|
PEG10 DMR (1st) |
5'GGATATGAGTTATAGATTAATTT3' |
– |
45 |
| 5'CACTCATCAATCTATAATTCATA3' |
|
PEG10 DMR (2nd) |
5'GTAGATGTGTTGTAAAGTGATATT3' |
267 |
47 |
| 5'ACAATTCATACTAATTTCAATAC3' |
|
SNRPN DMR (1st) |
5'GGAAAGTTTGAGGAAATTTGAATAAGG3' |
– |
55 |
| 5'CAAATACCCCCAAAACCTAACAAAA3' |
|
SNRPN DMR (2nd) |
5'TTGGGAGGTATTATTTTGGGTTGAAG3' |
548 |
55 |
| 5'AAAAAATCAATCCAACCCCAAACCTC3' |
|
XIST DMR (1st) |
5'GGGTGTTTTTGTTTTAGTGTGTAGTA3' |
– |
51 |
| 5'CTTTAATACCACCCACTAAAATTAATAC3' |
|
XIST DMR (2nd) |
5'TTGTTATATAGTAAAAGATGGT3' |
405 |
46 |
| 5'ACCAATCCTAACTAACTAAATA3' |
|
NANOG DMR (1st) |
UAGAGTGAATTAAAGAGGAAAATGG3' |
– |
51 |
| 5'TATAAAAATAAAAACCATCCAATCCA3' |
|
NANOG DMR (2nd) |
5'GTAGTTTTTGTTATAAATTAGTTTGA3' |
361 |
51 |
| 5'AAATAAAACTCAACCATACTTAACC3' |
|
OCT4 DMR (1st) |
5'GGGTGGAGAGTAATTTTGAGGG3' |
– |
51 |
| 5'TAATACTAACTAATAATAAATAACC3' |
|
OCT4 DMR (2nd) |
5'GAAGTTGGATAAGGAGAAGTTGGAG3' |
316 |
51 |
| 5'AATAAAAAAACCTACTTAACAAAAACC3' |
