Fig. 2.

Effects of AGEs on IL-1β production in the Sw.71 human placental cell line. (A and B) Sw.71 cells were incubated for 24 h with BSA or AGEs (200 μg/ml), and LPS (100 ng/ml); The IL-1β mRNA levels were measured using qRT-PCR. Pro-IL-1β and ACTB protein levels were subsequently quantified in the cell lysates by western blot. (C–F) After incubation, the NLRP3, ASC and CASP1 mRNA levels were measured using qRT-PCR. Significant differences were identified using ANOVA, followed by Bonferroni’s multiple comparison test; * P < 0.05 and ** P < 0.01.