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. 2017 May 18;63(4):401–408. doi: 10.1262/jrd.2017-032

Fig. 5.

Fig. 5.

ROS pathways of AGEs and their effect on inflammatory cytokine production in the Sw.71 human placental cell line. Sw.71 cells were pre-incubated for 1 h with specific pharmacological inhibitors (NAC as an antioxidant, DPI as a NADPH inhibitor). (A) After inhibitor treatments, cells were incubated for 24 h with AGEs (200 μg/ml), and then treated with nano-silica (100 μg/ml) for 24 h. IL-1β levels in the supernatant were determined using ELISA. (B) After NAC treatments, cells were incubated for 24 h with AGEs (200 μg/ml). The IL-1β mRNA levels were measured using qRT-PCR. Data are expressed as mean ± SEM (n = 4). Significant differences were identified using ANOVA, followed by Bonferroni’s multiple comparison test; * P < 0.05.