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. 2017 May 27;63(4):409–414. doi: 10.1262/jrd.2016-179

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©2017 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 5. — Hypothalamic (A, B, C) and ovarian (D, E, F) Kiss1 mRNA levels 3 h after saline injection, or 3 h or 6 h after HCG administration in rats on days (D) 10 (A, D), D20 (B, E), and D30 (C, F) (n = 7 per group). The mRNA levels in the hypothalamus are expressed as values relative to GAPDH, and those in the ovary are expressed as values relative to β-actin. The mRNA levels of the saline-injected groups were set as 1.0. Data are presented as the mean ± SD. a, b: different letters indicate different significant differences (P < 0.05).