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. Author manuscript; available in PMC: 2018 Apr 17.
Published in final edited form as: Chem Res Toxicol. 2017 Mar 22;30(4):1093–1101. doi: 10.1021/acs.chemrestox.6b00457

Figure 1.

Figure 1

HPLC detection of retene metabolites in human HepG2 cells. (A) UV chromatogram at λmax 258 nm at 0 h. (B) UV chromatogram at λmax 258 nm at 24 h. (C) FLR chromatogram at λex 259 nm and λem 370 nm at 0 h. (D) FLR chromatogram at λex 259 nm and λem 370 nm at 24 h. Human HepG2 cells (~5 × 106) were treated with retene (1 μM, 0.2% (v/v) DMSO) in MEM (without phenol red) containing 10 mM glucose. The cell media were collected at 0 and 24 h, respectively, and subsequently acidified with 0.1% formic acid before extraction with ethyl acetate. The extracts were analyzed by HPLC-UV-FLR detection.