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Motility after thawing (%) of sperm from Xiphophorus helleri when suspended in Hanks’ balanced salt solution (HBSS) at 300 mOsm/kg and loaded in 0.25-ml French straws in different volumes
| Fish number | Volume (μl)
|
|||
|---|---|---|---|---|
| 30 | 60 | 100 | 200 | |
| 1 | – | 10 | 10 | 10 |
| 2 | 10 | 20 | 25 | 25 |
| 3 | 30 | 15 | 20 | 20 |
| 4 | 15 | 15 | 15 | 15 |
| 5 | 20 | 30 | 25 | 25 |
| Mean ± S.D. | 19 ± 9 | 18 ± 8 | 19 ± 7 | 19 ± 7 |
