Fig 5. STORM analyses of PBCV-1 infected cells reveal condensed cytoplasmic viral DNA.

Cells were infected for 1.5–2 min and processed for immuno-florescence with anti-PBCV-1 capsid antibodies, counterstained with SYTOX Orange for DNA detection and analyzed by STORM. A. A STORM image showing a host cell with two capsids at its periphery (yellow). Inset shows a virus delivering its genome (green) into the host cytoplasm at the opposite side of the nucleus. B. Another chlorella cell with an adjacent virus particle in the process of delivering its DNA into the nucleus (white arrowhead). In both panels the dashed line marks the nuclear boundary. Nu: nucleus. Scale bars: 1 mm.