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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Mar 29;1861(7):1825–1832. doi: 10.1016/j.bbagen.2017.03.020

Figure 3. Binding analysis of R1.2 with BJAB cells in the presence of 1µM sIgM.

Figure 3

Antigen specificity of R1.2 was further analyzed by cell binding assay in the presence of sIgM. BJAB cells (0.5 × 105) were incubated for 45min in the presence of human soluble IgM (48.5 µg per tube), or the same amount of BSA as a control, against three different FAM-dT-labeled R1.2 concentrations (500nM, 250nM and 125 nM). Cells were then washed once using 1 ml of wash buffer, followed by analysis of R1.2 binding by flow cytometry. Bar diagram is the overall conclusion from two independent experiments. Asterisks represent adjusted p values from Sidak’s multiple comparisons test based on comparing BSA vs. sIgM data for each concentration. (**: P ≤ 0.01; *** : P ≤ 0.001)