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. 2017 Sep 6;10:4355–4367. doi: 10.2147/OTT.S141239

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© 2017 Weagel et al. This work is published and licensed by Dove Medical Press Limited

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Flow cytometry analysis of Raji cells.

Notes: (A) Controls used for flow cytometry analysis of Raji cells. Cells are NK-κB− and CD19⁺. (B) Histograms and density plots showing a fluorescence intensity shift in Raji cells stained with anti-TK1 antibodies, A72, A74, and CB1. Cells show a significant fluorescent shift when bound to anti-TK1 antibodies. Isotype control and NFκB fluorescent shifts are nonsignificant, suggesting low nonspecific binding and integrity of the cell membrane. (C) Quantification of the percentage of cells showing a positive fluorescent shift. Raji cells show significant fluorescent shift when bound to anti-CD19 and anti-TK1 antibodies when compared to controls. *P≤0.05; **P≤0.01; ***P≤0.001.

Abbreviation: FITC, fluorescein isothiocyanate.

Flow cytometry analysis of Raji cells.

Notes: (A) Controls used for flow cytometry analysis of Raji cells. Cells are NK-κB− and CD19⁺. (B) Histograms and density plots showing a fluorescence intensity shift in Raji cells stained with anti-TK1 antibodies, A72, A74, and CB1. Cells show a significant fluorescent shift when bound to anti-TK1 antibodies. Isotype control and NFκB fluorescent shifts are nonsignificant, suggesting low nonspecific binding and integrity of the cell membrane. (C) Quantification of the percentage of cells showing a positive fluorescent shift. Raji cells show significant fluorescent shift when bound to anti-CD19 and anti-TK1 antibodies when compared to controls. *P≤0.05; **P≤0.01; ***P≤0.001.

Abbreviation: FITC, fluorescein isothiocyanate.