Figure 4.

Flow cytometry quantification analysis of normal lymphocytes.

Notes: (A) Lymphocytes were isolated from whole blood and stained with anti-TK1 antibodies, A72, A74, and CB1, anti-CD45 (positive control), isotype control, and anti-NFκB antibodies. Lymphocytes were also stimulated to proliferate and then stained with anti-TK1 antibodies, A72, A74, and CB1, anti-CD45 (positive control), isotype control, and anti-NFκB antibodies. Normal resting lymphocytes show the absence of TK1 on their surface even under proliferating conditions. (B) Lymphocytes were treated with supraphysiological levels of purified TK1 (0.25 µM, 0.5 µM, and 0.75 µM TK1). Lymphocytes were stained with anti-TK1 antibody (ab91651) and CD52 (positive control). Lymphocytes show nonsignificant amounts of TK1 on their surface, suggesting that extracellular sources of TK1 do not interact with the membrane. ***P≤0.001; ****P≤0.0001; ns= P>0.05, non significant.