Skip to main content
. 2017 Aug 30;40(4):1143–1151. doi: 10.3892/ijmm.2017.3113

Figure 1.

Figure 1

Osthole suppresses the viability and colony formation of intrahepatic cholangiocarcinoma (ICC) cells. (A) The chemical structure of osthole. (B) A cell counting kit-8 (CCK-8) assay was performed to analyze the viability of ICC cells treated with various concentrations of osthole (0, 25, 50, 100, 150 or 200 μM) for 24, 48 or 72 h. (C) ICC cells were treated with 150 μM osthole for 24, 48 or 72 h. Then, cell viability was assessed using the CCK-8 assay. (D and E) Cells were cultured with various concentrations of osthole (0, 25, 50, 100, 150 or 200 μM) and were allowed to form colonies for 14 days. *P<0.05 and **P<0.01 vs. control.