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. 2017 Aug 30;40(4):1143–1151. doi: 10.3892/ijmm.2017.3113

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Copyright: © Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Osthole induces mitochondrial-dependent apoptosis in RBE and HCCC-9810 cells. (A) The mitochondrial membrane potential (ΔΨm) was measured using flow cytometric analysis. RBE and HCCC-9810 cells were treated with osthole (0, 50, 150 or 200 μM) and then stained with rhodamine 123. Cells with high ΔΨm are marked 'survival', and those with low ΔΨm are marked 'apoptosis'. (B) Apoptosis-related proteins in RBE and HCCC-9810 cells were analyzed by western blotting. GAPDH was used as a loading control. ^*P<0.05 and ^**P<0.01 vs. control.