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Function-related protein expression in passaged HCECs cultured with 4 µM TZV for different periods, n=3. (A) E-cadherin, N-cadherin, and α-SMA immunostaining (magnification ×200); (B) ZO-1 immunostaining; (C) Na+/k+-ATPase immunostaining. DMSO served as the negative control, and the concentration was 0.6‰; Y-27632 served as the positive control, and the concentration was 10 µM. HCECs, human corneal endothelial cells; TZV, thiazovivin.
