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. 2017 Aug 16;40(4):1165–1171. doi: 10.3892/ijmm.2017.3100

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Copyright: © Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Transforming growth factor-β1 (TGF-β1) upregulates the expression of p-Par6 in rat NRK-52E cells. NRK-52E cells were untreated (control) or incubated with TGF-β1 (20 ng/ml) for 72 h. (A) The mRNA expression of Par6 was measured by RT-PCR. (B) Representative western blots of Par6 and p-Par6 in control cells and TGF-β1-stimulated cells. The histogram shows the average volume density corrected for the loading control, GAPDH. (C) Par6 was visualized by confocal fluorescence microscopy (×600 magnification). Values are expressed as the means ± SEM, n=3 in each group. ^*P<0.05 vs. control group.