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. 2017 Aug 3;40(4):1125–1133. doi: 10.3892/ijmm.2017.3089

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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mRNA and protein expression level sof Bcl-2 associated gene X (Bax), B cell lymphoma gene-2 (Bcl-2) and caspase-3 as determined by RT-qPCR and western blotting in the PC12 cells after 48 h hypoxia. (A) The mRNA expression levels of Bax, Bcl-2 and caspase-3 by RT-qPCR. (B) The protein expression levels of Bax, Bcl-2 and caspase-3 by western blotting. The images indicate that after 48 h hypoxia, the mRNA and protein expression levels of Bax and caspase-3 were significantly increased, and also obviously decreased after transfection of pCMV-Myc-Nova1, and that of Bcl-2 was significantly decreased, and also obviously increased after transfection of pCMV-Myc-Nova1 (^*p<0.05; ^**p<0.01).