Figure 7. Baclofen inhibits nocifensive behavioral responses induced by the TRPM3 channel agonist CIM0216, but not responses to the TRPA1 agonist AITC.
(A–B) Nocifensive responses to the injection of CIM0216 (50 nmol/paw) were recorded as described in Materials and methods in control animals, and in animals where 12.5 nmol/paw baclofen was also injected in the same hind paw. (A) Duration of licking, (B) number of licking (n = 13 for both groups). (C, D) Nocifensive responses to hind paw injection of 100 nmol/paw AITC were recorded as described in Materials and methods in control animals, and in animals where 12.5 nmol/paw baclofen was co-injected. (C) Duration of licking, (D) number of licking (n = 12 for AITC and n = 11 for AITC + baclofen). Statistical analysis was performed with two sample t-test *p<0.05, **p<0.01, ns: p=0.5 (C) and p=0.63 (D).