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. 2017 Jul 26;8(34):55821–55836. doi: 10.18632/oncotarget.19608

Figure 3. Effect of p53 knockdown on the regulation of common p53-repressed genes.

Figure 3

At 24 hours after transfection of the indicated siRNA, HBC4 cells were treated with 2 μg/ml ADR for 2 hours. Forty-eight hours after treatment, the mRNA expression level of the indicated genes was determined using qPCR and the log2-fold change of mRNA expression against the control condition (Mock, no ADR treatment) was calculated using the ΔΔCt method. The data are presented as the mean±SD from three independent experiments. The red dotted line shows cutoff value. *P < 0.05, **P < 0.01, ***P < 0.001, N.S., not statistically significant. Representative data (9 genes) are presented. Additional data are shown in Supplementary Figure S4.