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. 2017 Aug 3;8(34):55848–55862. doi: 10.18632/oncotarget.19862

Figure 3. Elevated mitochondrial superoxide in p16-deficient cells is associated with enhanced cell migration.

Figure 3

a. Wild-type (WT) and p16−/− primary fibroblasts were stained with MitoSox-Red and assessed for mitochondrial superoxide by flow cytometry. Error bars indicate SEM from triplicate determinations, *P < .001. Representative histogram at right. b. Detection of superoxide in the absence (0 h) or presence of 5 mM N-acetylcysteine (NAC) after 1 or 24 h. Error bars indicate SEM from triplicate determinations, *P < .001, *P = .006. c. Western blots of lysates from cells in a.. d. Wild-type (WT) and p16−/− fibroblasts were subjected to transwell migration assay, in the absence or presence of 5 mM NAC, and migrating cells were counted at 24 h. Error bars indicate SEM from triplicate determinations, *P = .003. e. Migration assay as in d., with cells in absence or presence of 50 μM CCCP. Error bars indicate SEM from triplicate determinations, *P = .005.