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. 2017 Aug 3;8(34):55848–55862. doi: 10.18632/oncotarget.19862

Figure 5. Forced expression of p16 modulates mitochondrial biogenesis, respiration, and motility.

Figure 5

a. A375 human melanoma cells were infected with control (GFP) or GFP/p16-expressing lentivirus, then 6 d later lysates were subjected to Western blotting. b. Cells in a. analyzed for mitochondrial superoxide by flow cytometry. Error bars indicate SEM from duplicate determinations, *P < .001. c. Maximal respiration of cells in a.. Error bars indicate SEM from 9 determinations, *P = .04. d. A375 cells were infected with control (GFP) or GFP/p16-expressing lentivirus, then 48 h later were placed in migration assay, and migrating cells counted at 24 h. Error bars indicate SEM from triplicate determinations, *P < .001. e. Human melanocytes were infected with control (GFP) or GFP/p16-expressing lentivirus, then 6 d later lysates were subjected to Western blotting. f. Cells in e. analyzed for mitochondrial superoxide by flow cytometry. Error bars indicate SEM from duplicate determinations, *P < .001. g. Maximal respiration of cells in e.. Error bars indicate SEM from 9 determinations, *P < .001. h. Melanocytes were infected with control (GFP) or GFP/p16-expressing lentivirus, then 48 h later were placed in migration assay and migrating cells counted at 48 h. Error bars indicate SEM from triplicate determinations, *P = .003.