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. 2017 Aug 3;8(34):55848–55862. doi: 10.18632/oncotarget.19862

Figure 7. CDK4 knockdown does not mimic effects of p16 on mitochondrial dynamics.

Figure 7

a. YU2 melanoma cells were transfected with control or RNAi specific for CDK4, then lysates were analyzed by Western blotting 72 h later. b. Cells in a. analyzed for mitochondrial superoxide by flow cytometry. Error bars indicate SEM from triplicate determinations; NS, not significant. c. Cells in a. analyzed for mitochondrial mass by flow cytometry. Error bars indicate SEM from triplicate determinations; *P < .001. d. Human melanocytes were transfected with control or RNAi specific for CDK4, then lysates were analyzed by Western blotting 72 h later. e. Cells in d. analyzed for mitochondrial superoxide by flow cytometry. Error bars indicate SEM from triplicate determinations; *P = .04. f. Cells in d. analyzed for mitochondrial mass by flow cytometry. Error bars indicate SEM from triplicate determinations; *P = .04.