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. 2017 Aug 3;8(34):55863–55876. doi: 10.18632/oncotarget.19866

Figure 1. N-terminal truncations of p85.

Figure 1

A. Schematic representation of the p85 N-terminal truncation mutants. B. Representative focus assays of p85 N-terminal truncations. C. Activation of PI3K signaling in cells expressing N-terminally truncated p85 as reflected by Western blots of Akt phosphorylated at S437 and S6 at Ser235/236. The data in Figure 1C are from a representative experiment that was repeated three times with consistent results (for details, see the Materials and Methods section). D. Efficiencies of cellular transforming activity of p85 N-terminal truncation mutants relative to the transforming activity of wild type p85β. CEF cells were transfected RCAS(A) constructs expressing the inserts p85β, p85β ΔSH3, p85β ΔSH3-RhoGAP, p85α, p85α ΔSH3, and p85α ΔSH3-RhoGAP. Transforming activity was standardized to 0.5 μg transfected DNA.