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. 2017 Jun 3;8(34):55967–55983. doi: 10.18632/oncotarget.18356

Figure 5. Melatonin inhibited Sirt1-upregulated NFAT5 expression in IL-1β-challenged chondrocytes.

Figure 5

A. IL-1β-exposed chondrocytes were pretreated with or without 100 nM siSirt1 and incubated in serum-free media overnight, and ChIP assay was performed. B.-D. Chondrocytes were pretreated with 100 μM Sirt1 inhibitor (EX527) for 30 min or 100 nM siSirt1 for 1 h and then stimulated with or without 10 ng/ml IL-1β for 30 min, followed by 10 ng/ml melatonin for 24 h. (B) qPCR and (C and D) Western blot analyses were performed to determine the mRNA and protein expression of NFAT5, respectively. GAPDH and β-actin were used as internal controls. Each value represents mean ± SD of 3 replicates or representative of 3 independent experiments. *P < 0.05 compared with control; #P < 0.05 compared with IL-1β-treated group; §P < 0.05 compared with Siscrb group. Mel: melatonin.