Skip to main content
. 2017 May 23;8(34):56110–56125. doi: 10.18632/oncotarget.18125

Figure 2. SAHA interferes with nuclear translocation of HIF-1α and HIF-2α.

Figure 2

A. Hep3B cells were cultured under normoxic or hypoxic conditions for 16 hr. Representative western blot with normalized densitometric values (protein/actin loading control) show no change in total cellular HIF-1/2α expression at low concentrations of SAHA (0.1 μM, +), and moderate decrease at 2 μM (++). B. Representative western blot with normalized densitometric values (protein/histone loading control) show nuclear proteins isolated from Hep3B cells exposed to 0.1 μM (+) SAHA for 16 hr under indicated conditions with decreased nucleated HIF-1α and HIF-2α. C. Luciferase reporter assay demonstrates a significant decrease in HRE-associated luciferase activity in response to SAHA under hypoxic conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.