Table 2. Thermodynamic parameters extracted from the calorimetric evaluation of unphosphorylated Δ574–580, Δ559, and T670I mutant KITs titrated Imatinib.

KIT mutant	ΔGbind,ITC (kcal/mol)	ΔHbind,ITC (kcal/mol)	-TΔSbind,ITC (kcal/mol)	Kd,ITC (nM)	n
Δ574−580	−8.80	−10.73	1.93	359	1.12
Δ559	−9.41	−11.67	2.26	127	1.09
T670I	−6.18	−7.25	1.07	29450	0.96

Experiments were performed in triplicate at 25°C. Standard deviation values are within 1–3% for ΔH_bind,ITC, 3–5% for K_d,ITC, and 1–2% for n