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. 2017 Apr 20;8(34):56228–56242. doi: 10.18632/oncotarget.17280

Figure 3. HCV core variants mediate 4E-BP1 phosphorylation in transgenic mouse livers.

Figure 3

(A) Liver proteins extracted from 9-month-old WT, transgenic mice that specifically express HCV core protein isolated from tumor and non-tumor (cT or cNT) areas (4 different mice per group) were analyzed for phospho 4E-BP1 by immunoblot. (B) Quantification of phospho-4E-BP1 relative to 4E-BP1 levels, *p value < 0.05. (C) Immunohistochemical staining of WT, cT and cNT liver biopsies. Liver slices were immunostained with p4E-BP1 antibody and representative results are shown (magnification x40). (D) Protein lysates of primary mouse hepatocytes isolated from transgenic mouse livers expressing or not cT and cNT were analyzed by immunoblot with antibodies directed against phospho 4E-BP1. One representative experiment is shown and p38 is used as loading control. (E) Depiction of normalized densitometric values of phospho-4E-BP1 over 4E-BP1 in primary mouse hepatocytes, *p value < 0.05.