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. 2017 Apr 20;8(34):56228–56242. doi: 10.18632/oncotarget.17280

Figure 6. HCV core increases 4E-BP1 phosphorylation level in Myc/cT and Myc/cNT mouse livers.

Figure 6

(A) Representative immunoblotting assay of phospho 4E-BP1 on proteins extracted from livers of 3-month-old simple Myc/WT or double transgenic, Myc/cT and Myc/cNT mice (4 different mice/group). (B) Average fold changes of densitometric values of phospho Thr37/46 4E-BP1 expression relative to 4E-BP1 levels are represented graphically, *p value < 0.05. (C) Immunohistochemical analysis of tissue slices from paraffin-embedded livers of simple Myc/WT or double transgenic mice, Myc/cT and Myc/cNT, using phospho-4E-BP1 (magnification x40). (D) Protein extracts from double transgenic liver tumors (T) and the adjacent non-tumor tissues (NT) of 9-month-old Myc/WT, Myc/cT and Myc/cNT were analyzed for phospho-4E-BP1. One representative immunoblot of three independent experiments is shown, p38 is used as loading control.