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. 2017 Apr 27;8(34):56255–56266. doi: 10.18632/oncotarget.17447

Figure 4. Knockout of FoxR2 inhibits the migration and invasion of glioma cells.

Figure 4

(A) Cell migratory ability of different groups were detected by wound healing assay in U251 cells. (B) Quantitative analysis of migratory cell numbers. (C) Cell migratory ability after FoxR2 knockout was assessed by transwell invasion assay. (D) Quantitative cell numbers invaded through the filter. The numbers of migratory or invading cells were normalized to the control-sgRNA group. (E) Representative picture and quantitative MMP-2 activity of gelatin zymography assay. FoxR2-sgRNA2 and control cells were starved in serum-free DMEM for 24 h, then gelatin zymography assay was performed. (F) The effects of FoxR2 knockout on MMP-2 protein levels by western blot analysis. (G) The effects of FoxR2 knockout on mRNA levels of MMP-2 by Real time PCR analysis. The above results are expressed as the mean ± SEM from three independent experiments, *P < 0.05, **P < 0.01.